Software and data packages

Open Case Studies data analyses

Bioconductor

Software

  • R/mbkmeans : R/Bioconductor package implementing the mini-batch optimization for k-means clustering proposed in Sculley (2010) for large datasets, including single-cell RNA-sequencing data. The mini batch k-means algorithm can be run with data stored in memory or on disk (e.g. HDF5 file format).
  • R/qsmooth : R package available that implements a generalization of quantile normalization, referred to as smooth quantile normalization (qsmooth), which is based on the assumption that the statistical distribution of each sample should be the same (or have the same distributional shape) within biological groups or conditions. (Hicks et al., 2018. Biostatistics)
  • R/quantro : R package available on Bioconductor to test for global differences between groups of distributions to decide when to use quantile normalization. (Hicks and Irizarry, 2015. Genome Biology)

Data packages

  • benchmarkfdrData2019: Benchmarking results for experimental and simulated data sets used in Korthauer and Kimes et al. (2019) to compare methods for controlling the false discovery rate. A shiny app to interactively explore the data is available on the benchmark-shiny repository on GitHub.
  • bodymapRat: R data package that contains an SummarizedExperiment from the Yu et al. (2013) paper that performed the rat BodyMap across 11 organs and 4 developmental stage.
  • TENxPBMCData: R data package that contains a set of SingleCellExperiment objects with single-cell RNA-sequencing data from peripheral blood mononuclear cells generated by 10X Genomics.

GitHub

Software

  • R/methylCC: R package available to estimate the cell composition of whole blood in DNA methylation samples in microarray or sequencing platforms
  • R/quantroSim: Supporting data simulation R-package for the quantro R-package to simulate gene expression and DNA methylation data.
  • R/explainr: translates S3 objects into text using standard templates in a simple and convenient way.
  • postMUT: A tool implemented in Perl and R to predict the functionality of missense mutations.

Data packages

  • trapnell2014myoblasthuman: R data package that contains an ExpressionSet object from Trapnell et al. (2014) that performed a time-series experiment of bulk and single cell RNA-Seq at four time points in differentiated primary human myoblasts.
  • patel2014gliohuman: R data package that contains a SummarizedExpression object from Patel et al. (2014) with single cell and bulk RNA-Seq data on five human glioblastoma tumors.
  • colonCancerWGBS: Cov files produced from Bismark after mapping six paired tumor-normal WGBS samples from Ziller et al. (2013) PMID: 23925113. Only chr22.
  • myAffyData: AffyBatch object from an experiment using P493-6 cells expressing low or high levels of c-Myc. Data from Loven et al. (2012) Cell 151: 476-482.
  • BackgroundExperimentYeast: AffyBatch object from an experiment to measure NSB and optical noise in yeast.